The Confocal Raman Spectroscopy in vivo is an effective tools for Penetration Process Analysis of Alpha-tocopheryl acetate in the stratum corneum. Different concentrations of the vitamin E derivative are found on the SC, depending on the area and depth of the body examined, showing the process of penetration.

By confocal Raman, the physical sectioning of hair fibers is not required to obtain molecular chemistry and structure information within the fiber. This provides opportunities for the nondestructive examination of hair fibers under many different conditions.

The confocal Raman spectroscopy technique has the advantage of providing detailed information on the composition and molecular skin structure, besides being possible to measure the skin moisture content through the calculation of the water and NMF concentration profile.

Confocal Raman Spectroscopy is a method for determining the permeation of active to the skin. It is important to improve the transdermal delivery and bioavailability of the active.

Abstract of the presentation.